Wondfo Pro DX Canine Progesterone Testing – Comprehensive Training Guide

Welcome to the Wondfo Pro DX Training Page, your go-to resource for mastering the use of this advanced immunofluorescence machine. With results in just 15 minutes, the Wondfo Pro DX is designed to give breeders fast, accurate progesterone testing to ensure optimal breeding timing.

Step-by-Step Guide to Using the Wondfo Pro DX

1. Prepare Your Blood Sample:

   • Serum Testing:
     Draw blood from your canine patient and spin it down to isolate the serum. Ensure the serum is clear and not pink or red, as hemolyzed serum can affect the accuracy of your results.

   • Whole Blood Testing:
     Skip the spinning step! Just pipette 75 µL of whole blood directly into the buffer tube provided in your test kit.

2. Mix the Serum/Buffer or Blood/Buffer:

   • Once you have your 75 µL of serum (or whole blood for whole blood testing), pipette it into the buffer tube.

   • Slowly mix by turning the buffer tube up and down 8-10 times to ensure the sample is well combined with the buffer.

3. Load the Test Cartridge:

   • Pipette 75 µL of your buffer-serum mixture (or buffer-blood mixture) into the small well of the test cartridge.

4. Incubate the Sample:

   • If you're using the Fincubator, ensure it's preset to 78°F with the timer set to 15 minutes. Insert the test cartridge, and once the time is up, the results will be ready for reading.

5. Read the Results:

   • After incubation, run the test on the Wondfo Pro DX. The results will be displayed in ng/mL (nanograms per milliliter) on the screen, and you can also print the results using the built-in printer.

Breeding Timing and Progesterone Levels Explained

Using the Wondfo Pro DX machine helps you pinpoint the optimal time to breed your dog. Here’s a breakdown of progesterone levels and what they mean:

• LH Surge:
  Progesterone levels between 2-5 ng/mL indicate the luteinizing hormone (LH) surge, a critical point when ovulation is imminent.

• Ovulation:
  When progesterone reaches around 15 ng/mL, ovulation occurs. Breeding should happen shortly after this point, as the eggs will begin to mature.

• Breeding Window:
  Once progesterone reaches 15 ng/mL and ovulation has occurred, the ideal breeding window will continue as progesterone levels rise. Generally, you should aim for levels between 5-40 ng/mL to time your breedings for success.

Watch These Training Videos:

1. How to Use the Wondfo Pro DX Progesterone Machine
   This video offers a hands-on tutorial on using the Wondfo Pro DX machine, from sample prep to running the test and reading results.

2. When to Breed Your Dog and Breeding Timing
   Learn the optimal breeding window based on progesterone levels and maximize your chances for successful breedings.

3. Common Mistakes in Progesterone Testing
   Avoid common testing mistakes to ensure accurate results every time.

Testing Options: Serum vs. Whole Blood

The Wondfo Pro DX offers flexibility in testing by allowing both serum and whole blood tests. Here’s a comparison:

• Serum Testing:
  Requires spinning the blood to separate serum. This method provides very precise results and is recommended for most cases.

• Whole Blood Testing:
  No spinning required! Simply pipette 75 µL of whole blood into the buffer tube and follow the same mixing and loading steps as serum testing. This is a convenient option when you’re short on time or don’t have access to a centrifuge.

Built-In Features of the Wondfo Pro DX

• No Calibration Needed:
  The Wondfo Pro DX does not require manual calibration, as the calibration information comes with each new box of test kits on the ID chip.

• Built-In Printer:
  Print results instantly for easy record-keeping and client communication.

• Long Battery Life:
  The Pro DX machine features a lithium-ion battery that can perform up to 800 tests on a single charge, ensuring you have plenty of power during busy breeding periods.

Common Testing Mistakes and How to Avoid Them

Accurate testing is key to successful breeding. Here are a few tips to avoid common pitfalls:

1. Avoid Hemolyzed Samples:
   Hemolyzed (pink or red) serum can lead to inaccurate results. Ensure your sample is clear before proceeding.

2. Proper Mixing of Buffer and Sample:
   Always mix your buffer and sample gently by turning the buffer tube up and down 8-10 times. Avoid shaking it too vigorously, as that can introduce bubbles and affect the result.

3. Use the Correct Test Kit:
   Make sure you're using the correct test kit for either serum or whole blood testing, depending on your method.

Lifetime Customer Support

When you purchase a Wondfo Pro DX machine from K9Reproduction, you’re not just getting a top-tier progesterone testing machine—you’re getting access to lifetime customer support. Our team is here to assist you with any questions or troubleshooting, ensuring you get the most accurate and reliable results for your breeding program.

For further details, feel free to explore our additional resources or reach out to us for support. At K9Reproduction, we’re committed to helping breeders achieve success with the best tools and guidance.